A Mutation That Supercharges Tumour-Suppressor
Cancer researchers have long hailed p53, a tumour-suppressor protein, for its ability to keep unruly cells from forming tumours. But for such a highly studied protein, p53 has hidden its tactics well.
Now, researchers at the Stanford University School of Medicine have tapped into what makes p53 tick, delineating a clear pathway that shows how the protein mediates anti-tumour activity in pancreatic cancer. The team’s research also revealed something unexpected: A particular mutation in the p53 gene amplified the protein’s tumour-fighting capabilities, creating a “super tumour suppressor.”
The protein functions a bit like a puppet master in the genome, guiding the activation or suppression of many cancer-relevant genes in the body. “But if you simply ask how cells with and without p53 are different, you’ll see that there are at least 1,000 genes whose expression is affected by p53 status,” said Laura Attardi, PhD, professor of radiation oncology and genetics, and senior author of the study. “So, getting to the bottom of which of those many genes are critical to tumour suppression is not a trivial question.”
The paper describing the work was published online in Cancer Cell, where research associate Stephano Mello, PhD, is the lead author.
Mutated for the Better
Attardi began sorting out the puzzle by testing the effect of several individual p53 mutations in mice that were predisposed to pancreatic cancer. Any change in p53 activity typically points to trouble: Too little leaves the body susceptible to tumour growth, whereas too much can cause problems in development. But surprisingly, one of the p53 mutants actually kept the mice tumour-free longer, suggesting it was a super version of p53.
“What’s incredible about this mutant is that it hit a sweet spot,” Attardi said. “Embryos can make it through development without any obvious effects, and then adult mice show greatly enhanced resistance to tumour growth.”
Mice that harboured the favourable mutation, which occurred in a transcriptional activation domain called TAD2, displayed longer, pancreatic cancer-free survival than mice with normal copies of the p53 gene. Attardi’s study showed that, at 400 days old, nearly 40 percent of the mice with normal p53 function had succumbed to pancreatic cancer, whereas none of the mice with the mutant form showed signs of tumour formation.
“It’s not to say that mice with the mutated version of p53 would never get cancer, but this experiment suggests that this particular mutant is really potent in limiting tumour development,” Attardi said.
It turned out that the mutant hyperactivates p53, causing a subset of its downstream targets to get a surge of activity, too. But with more than 100 target genes sent into overdrive, it was critical for Attardi’s team to narrow down which genes directly affected tumour suppression. Genomic data and past studies in human cancers pointed the team to the gene Ptpn14. More importantly, Ptpn14 is a known regulator of Yap, a protein that, when unchecked, turns on cancer-promoting genes in the body.
The Axis of Tumour Super-Suppression
Attardi’s findings allowed her to define a pathway, or “axis,” consisting of three proteins that contribute to p53-mediated tumour suppression, and it works in a linear fashion. In the chain of command, p53 ranks highest and activates Ptpn14; Ptpn14 then suppresses Yap to keep cells from turning cancerous.
In collaboration with co-author Christina Curtis, PhD, assistant professor of medicine and of genetics, and postdoctoral scholar Jose Seoane, PhD, Attardi used human cancer genomic data to extend the paradigm further, showing that when p53 is mutated in human cancer, Yap activity increases, allowing tumours to develop.
Attardi said the axis actually suggests that p53 and Ptpn14 deficiency can promote the same consequence of Yap activation.
“I think this p53-Ptpn14-Yap axis is a central mechanism,” Attardi said. “P53 affects a lot of tumour-suppression processes, so if it influences a central protein like Yap, which also controls a lot of cancer processes, it can have widespread effects on cell behaviour.”
Attardi added that she would be hesitant to say that this is the one and only mechanism. “It would be too simplistic to think that this is absolutely the only pathway that’s involved in p53-mediated suppression of pancreatic cancer, so I suspect that there will be other contributions as well.”
The team’s findings could inform a new type of therapeutic, mimicking the p53 super-mutant to upregulate tumour suppression. It could also inform those who are developing therapeutic Yap inhibitors.
“Clearly, Yap is a very potent oncogene,” Attardi said. “And our study suggests that perhaps the focus should be on developing Yap inhibitors for tumours where p53 is gone — maybe it’s more critical in those cancers.”
Now, Attardi and her team are continuing to investigate whether their newly uncovered p53 mechanism holds true for a wide range of cancers, not just pancreatic.
“We want to know if this is a tissue-specific pathway and if this really is relevant for different tumour types,” Attardi said. “So we’re turning to experimental models to test that.”
Materials provided by Standford University Medical Center. Note: Content may be edited for style and length.