molecular scissors CRISPR cas9

Copenhagen Center for Glycomics has announced a collaboration with Desktop Genetics to analyse their novel CRISPR assay. 

The project has been designed to provide a deeper understanding of a novel method of identifying nuclease-generated mutations developed by the lab of Dr. Eric Paul Bennett, writes Technology Networks

The method is being used by CCG at the moment, as well as by its spin-out company, Glycodisplay Aps. Looking ahead, the improvements to this tool will be a driver for broader use in academia and industry. 

Known as Indel Detection by Aplicon Analysis (IDAA), the method will significantly reduce the workload of cell line editing by facilitating the initial screening of newly generated nuclease reagents, in addition to the subsequent generation of edited cell pools or clonal cell lines. 

In turn, it reduces the number of clones necessary to create a stable cell line and increases the ease with which they are screened. Desktop Genetics will then analyse CRISPR indel formation using their machine learning expertise. As an end goal, the team will be working with CCG to analyse a dataset of 800 guides designed on the DESKGEN Cloud platform to explore the capabilities of IDAA. 

“We realised that an in-depth analysis of the experimentally validated, unbiased and well defined-indel profiles generated by 800 unique Cas9 guides could contribute to improving the predictive value of guide RNA design algorithms,” said Dr. Bennett. “Therefore, Desktop Genetics, with its leading position in the field of genome editing bioinformatics, was chosen as a partner to undertake this extensive analytical task.”

Dr. Leigh Brody, added, “This co-investigation will enable researchers to extract much more information from the IDAA assay by combining Eric’s and our teams’ expertise to deeply analyse these interesting datasets. This will provide a better understanding of CRISPR activity and, in turn, serve our broader goal of offering a comprehensive solution for genome editing analysis.”

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