Simplified Genome Editing and Detection Workflows
Genome editing is enabled by the development of tools to make targeted changes to the genome of living cells. Sequence-specific nucleases make double-stranded DNA breaks in genomic DNA. Breaks are repaired by the cellular DNA repair machinery to introduce small insertions and deletions in the absence of a repair template, or specific changes in the presence of a repair template.
Earlier approaches to genome editing relied on sequence specificity engineered into DNA binding domains of customized nucleases. These nucleases were costly and time-consuming to engineer, limiting their widespread use.
In contrast, CRISPR-Cas9 nucleases can be easily programmed to cut double-stranded DNA at almost any sequence by changing sequence contained in its guide RNA. This flexibility makes this system attractive for laboratory use and amenable to large-scale, high-throughput studies.
In this webinar you will learn how to increase editing efficiency by directly introducing S. pyogenes Cas9 ribonucleoproteins (RNPs) to cells through electroporation or lipofection. Rapid sgRNA synthesis requiring only a single user-supplied ~55mer single-stranded DNA oligonucleotide is described. Methods for assessing genome editing efficiency will be discussed including T7-endonuclease I-based methods, sequencing-based methods, and in vitro Cas9 digestion.
Join us for the webinar Simplified Genome Editing and Detection Workflows on September 12th at 9am PDT / 12pm EDT / 5pm BST.
Ezra Schildkraut, PhD works in the Product Development department at New England Biolabs with a focus on Genome Editing technology. Ezra Received his PhD in Biomedical Sciences from the University of New Mexico where he studied Recombination and DNA repair under the mentorship of Dr. Jac Nickoloff. Following graduate school, he worked in a CRO developing and purifying many products for New England Biolabs including many RNA modifying enzymes. Ezra joined NEB as a development scientist working on the RNA product line including In Vitro Transcription and messenger RNA products and eventually Genome Editing products. Ezra’s lab has worked in commercializing the Cas9 products as well as developing new genome editing tools such as the EnGen Mutation Detection kit and EnGen sgRNA Synthesis kit. Ezra’s lab continues to prioritize novel genome editing technologies with a commitment to the highest quality that NEB is known for.